These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Underestimation of D-glucose utilisation as judged from the conversion of D-[3-(3)H]glucose to (3)HOH.
    Author: Sener A, Giroix MH, Malaisse WJ.
    Journal: Diabetologia; 2002 Sep; 45(9):1274-80. PubMed ID: 12242460.
    Abstract:
    AIMS/HYPOTHESIS: The conversion of D-[3-(3)H]glucose to (3) HOH is currently measured to assess D-glucose utilisation. The validity of such a procedure was re-evaluated. METHODS: The conversion of D-[3-(3)H]glucose and D-[5-(3)H]glucose to (3) HOH was measured in rat pancreatic islets, parotid cells and erythrocytes. The tritiation of lipids were also examined in islets exposed to D-[3-(3)H]glucose or D-[5-(3)H]glucose. RESULTS: In rat pancreatic islets and parotid cells, but not in rat erythrocytes, the generation of (3) HOH from D-[3-(3)H]glucose underestimates the rate of D-glucose utilisation, this being apparently attributable to a partial escape from detritiation of [1-(3)H]glycerone-3-phosphate. Such an escape phenomenon resulted in a higher tritiation of lipids in pancreatic islets exposed to D-[3-(3)H]glucose, rather than D-[5-(3)H]glucose. Its relative extent was affected by a number of environmental factors such as the cell type under consideration, the metabolic status of the animals, and the extracellular concentration of D-glucose. CONCLUSION/INTERPRETATION: These findings impose a reservation on the use of D-[3-(3)H]glucose conversion to (3)HOH as a tool to assess the utilisation of the hexose in some cell types.
    [Abstract] [Full Text] [Related] [New Search]