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  • Title: Regeneration of flowering plants from difficile lily protoplasts by means of a nurse culture.
    Author: Horita M, Morohashi H, Komai F.
    Journal: Planta; 2002 Sep; 215(5):880-4. PubMed ID: 12244455.
    Abstract:
    The regeneration of difficile lily protoplasts isolated from suspension cells of the Oriental hybrid lily ( Lilium L.) cultivars Casablanca, Siberia and Acapulco was achieved by using the nurse-culture method. The divided protoplasts grew into colonies with nurse cells that have no regeneration ability, and developed to visible calli on a medium containing picloram. Many plantlets were formed on the calli after transfer of the proliferated calli to hormone-free medium. We were able to transplant the plantlets to soil in pots without acclimatization, and the plantlets grew in a greenhouse until flowering 2 years later.
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