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  • Title: [Effect of lyophilization methods on the formation of free radicals, the survivability and makeup of a population of the lincomycin producer].
    Author: Kuznetsov VD, Bushueva OA, Ruuge EK.
    Journal: Antibiotiki; 1975; 20(12):1065-8. PubMed ID: 1225179.
    Abstract:
    It was shown that a short-time exposure of the spores of Act. roseolus 981 to air (oxygen) on their lyophilization by the chamber method resulted in formation of increased numbers of the free radicals in the lyophilized spores suspension and decreased the survival of the spores on their further storage under vacuum. The phenomena were less pronounced when the exposure of the spores to the air was excluded by using the collector method of lyophilization. This testified to the advantages of the latter method as compared to the chamber one. No effect of the lyophilization methods on the population composition of the spontaneous variants of Act. roseolus 981 was observed.
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