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  • Title: Comparison of Chacko-Nair and Thayer-Martin media for culture of N. gonorrhoeae.
    Author: Siddappa K, Kushalappa PA, Inamadar AC, Vijayakumar GS.
    Journal: Indian J Sex Transm Dis; 1989; 10(2):57-9. PubMed ID: 12284233.
    Abstract:
    This article reports on a study designed to compare the efficacy and cost effectiveness of 2 selective media for culture of gonorrhea, the Chacko-Nair medium and the Thayer-Martin medium. The Chacko-Nair medium, an enriched egg=beef selective medium, is easily prepared from locally available ingredients in India. The Thayer-Martin medium, the standard selective medium used throughout the world, uses ready-made ingredients. In order to test the 2 mediums, researchers collected the urethral discharge of 240 male patients with acute gonorrhea and smear positive for N. gonorrhoea attending the Skin & STD, OPD of Chigateri General Hospital. All 240 specimens were subjected to culture study with the Chacko-Nair medium, and 190 were subjected to study with the Thayer-Martin Medium. The initial 30 cases under the Chacko-Nair medium did not show growth, since beef extract was used instead of fresh beef. Fresh beef was used in the remaining 210 samples, and they all showed profuse growth of organisms. All 190 sample under the Thayer-Martin medium showed good growth. While both mediums showed growth in 100% of the cases, the cost of the 2 differs considerably. Since all the ingredients are available locally, the Chacko-Nair medium is very cheap compared to the Thayer-Martin medium, whose ready-made ingredients are expensive and often difficult to procure. The Chacko-Nair medium, which can be prepared at any standard Microbiology Laboratory, has been used regularly at the Institute of Venerology in Madras, and has show results comparable to the Thayer-Martin medium. The report concludes that the Chacko-Nair medium is a good alternative to the Thayer-Martin medium.
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