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  • Title: [Ultrastructural cytochemistry of peroxidase and acid phosphat in mouse eosinophils].
    Journal: Haematologia (Budap); 1975; 9(3-4):209-17. PubMed ID: 1230406.
    Abstract:
    The distribution of peroxidase and acid phosphatase activity in the bone marrow of mouse eosinophils was investigated by electron microscopy. Peroxidase activity was found in the perinuclear space, the endoplasmic reticulum, the Golgi complex, non-mature and mature specific crystal-containing granules. In the course of development peroxidase activity disappears from the cisternal system. In mature eosinophils the enzyme is stored in specific granules, but some of these failed to reveal peroxidase activity. A hypothesis is offered concerning the complete condensation into crystal of the enzyme. In cells incubated in peroxidefree media some granular components stained weakly. Their activity probably depended on the presence of endogenous non-organic peroxidase. In developing eosinophils acid phosphatase was found in the Golgi complex and in non-mature specific granules. In the course of development, acid phosphatase disappears from the granules.
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