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  • Title: Use of the polymerase chain reaction to investigate the dynamics of pyrethroid resistance in Haematobia irritans irritans (Diptera: Muscidae).
    Author: Guerrero FD, Alison MW, Kammlah DM, Foil LD.
    Journal: J Med Entomol; 2002 Sep; 39(5):747-54. PubMed ID: 12349858.
    Abstract:
    A field study was conducted from 1991 through 1997 to evaluate the use of pyrethroid and organophosphate (OP) ear tags, alternated yearly, for the control of a pyrethroid resistant horn fly, Haematobia irritans (L.), population in Louisiana. Fly resistance was monitored by weekly fly counts, filter paper bioassays and diagnostic polymerase chain reaction (PCR) assays for the presence of pyrethroid resistance-associated mutations in the sodium channel gene coding region. Fly control in the first study year was poor, as pyrethroid ear tags were effective for only 7 wk. The following year, OP ear tags provided 15 wk of fly control. However, in all subsequent years, fly control was poor with both types of ear tags. The PCR assays showed that there were very few female flies homozygous for the pyrethroid susceptible sodium channel allele, never rising above 10% of the total females in the population. A fitness cost appeared to be associated with the pyrethroid resistant allele, as the resistant form was selected against in the absence of the pyrethroid ear tags. Despite this selection in favor of the susceptible allele and the annual alternation of pyrethroid and OP ear tags, the percentage of homozygous susceptible flies never reached over 19% of the population, resistant alleles of the sodium channel remained at high levels in the population, and horn fly control on cattle with either type of tag quickly became minimal.
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