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  • Title: Discelectrophoretic molecualr weight analysis of urinary proteins. A contribution to the clinical diagnostic differentiation and the pathophysiology of proteinuria.
    Author: Boesken WH.
    Journal: Contrib Nephrol; 1975; 1():143-55. PubMed ID: 1235087.
    Abstract:
    The SDS polyacrylamide gelelectrophoresis (SDS-PAA) as used in this study has proven to be an excellent tool to differentiate urinary proteins qualitatively and quantitatively, since the proteins are differentiated exclusively according to their molecular radius. Selectivity was estimated by the ratio transferrin:IgG. Some of the proteins were identified by specific antisera. For clinical use SDS-PAA may distinguish: chronic glomerulonephritis from chronic pyelonephritis; the different diabetic nephropathies; some cases of minimal change nephritis from proliferative and degenerative glomerular diseases; the uncomplicated posttransplantation course from (interstitial) rejection crises and from glomerular diseases (recurrent GN, glomerular rejection disease), and the persisting small glomerular proteinuria after acute glomerulonephritis from proteinurias becoming physiological.
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