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  • Title: Genetic evidence on the organization and action of the qa-1 gene product: a protein regulating the induction of three enzymes in quinate catabolism in Neurospora crassa.
    Author: Case ME, Giles NH.
    Journal: Proc Natl Acad Sci U S A; 1975 Feb; 72(2):553-7. PubMed ID: 123642.
    Abstract:
    The first three reactions in the catabolism of qainic acid in Neurospora crassa are under the genetic control of the qa gene cluster. This cluster consists of three structural genes encoding three inducible enzymes plus a regulatory gene (qa-1+) whose diffusible product apparently acts in a positive fashion to initiate coordinate synthesis of the three enzymes when an appropriate inducer is present. Genetic and biochemical evidence for both complementing and temperature-sensitive qa-1 alleles indicates that the product of the qa-1+ gene is an oligomeric (multimeric) protein. On the basis of cis-trans tests of appropriate double mutants (plus genetic mapping data for temperature-sensitive mutants), at least certain constitutive mutants (which produce all three qa enzymes in the absence of an inducer) are mutants in the regulatory gene and not in controlling elements such as initiators. The detection of stable (non-revertible) qa-1 intralocus deletion (multisite)mutants provides additional evidence for positive regulation in the qa system. Extensive genetic recombination data provide evidence that the two types of qa-1 mutants--slow-complementing (qa-1-s) and fast-complementing (qa-1-f)--map in discrete, non-overlapping segments of the qa-1 locus. These two distinct types of mutants are hypothesized to produce altered regulatory protein molecules that fail to interact either with a DNA initiator site (qa-1-s types) or with an inducer (qa-1-f types). The striking similarities between the qa system in this lower eukaryote and certain prokaryote operon systems are discussed.
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