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  • Title: Studies and analytical application of reaction of imipramine with chrome azurol S.
    Author: Starczewska B, Hałaburda P, Kojło A.
    Journal: J Pharm Biomed Anal; 2002 Oct 15; 30(3):553-60. PubMed ID: 12367680.
    Abstract:
    Chrome azurol S (CAS) was tested as a spectrophotometric reagent for the determination of imipramine (IMP). It reacts in aqueous media with IMP forming pink-red, sparingly soluble in water ion association compound. This compound was quantitatively extracted with chloroform and the absorbance of organic phase was measured at 510 nm. The extraction conditions were studied using a batch method. On the basis of the results obtained with batch method, three-line flow-injection system was constructed. Batch and flow-injection methods were successfully applied for the determination of IMP in pharmaceutical preparation.
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