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  • Title: [Application of multiplex PCR in genotyping of hepatitis B virus prevailing in Guangdong Province of China].
    Author: Yang J, Dai L, Guo YB, Yang SC, Wang YJ, Luo KX.
    Journal: Di Yi Jun Yi Da Xue Xue Bao; 2002 Aug; 22(8):707-9. PubMed ID: 12376255.
    Abstract:
    OBJECTIVE: To establish a convenient method for the genotyping of hepatitis B virus (HBV) using multiplex PCR. METHOD: Based on the alignment of 114 complete nucleotide sequences of HBV DNA belonging to different genotypes, acquired from the GenBank, genotype-specific sequences were identified according to which 6 pairs of primers were designed corresponding to each genotype. Subsequent genotyping of HBV was performed using these primers that were added, either alone or in conjunction with others, into a multiplex PCR reaction tube, and HBV genotype was determined according to the length of amplified DNA. RESULT: The genotyping result of multiplex PCR was consistent with that produced by PCR- restriction fragment length polymorphism as established by Lindh. We found in this study that among the HBV carriers in the vicinities Guangzhou of City, about 45% belonged to B genotype, 38.75% to C genotype and 16.75% to D genotype. CONCLUSION: This multiplex PCR method is simple, convenient and more differential.
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