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  • Title: Determination of L-sesamin and L-asarinin in Zanthoxylum(Roxb.) DC. by high performance liquid chromatography.
    Author: Zhang SY, Zhou BJ, Wang Y.
    Journal: Di Yi Jun Yi Da Xue Xue Bao; 2002 Jul; 22(7):654-5. PubMed ID: 12376305.
    Abstract:
    OBJECTIVE: To establish a method for determining the content of L-sesamin and L-asarinin indifferent parts of Zanthoxylum(Roxb.)DC. METHOD: High-performance liquid chromatography (HPLC) was utilized for this purpose with the column of HYPERSIL BDS C18 and the mobile phase containing acetonitrile and water (50:50), the detection wavelength being 287 nm. RESULTS: The average recovery rates for L-sesamin and L-asarinin were 100.13% and 1.61% and their relative standard deviation (RSD) were 101.24% and 1.46% respectively. CONCLUSION: The method can be used for the quality control of Zanthoxylum(Roxb.)DC, and the roots of this herb contains more L-sesamin and L-asarinin than its other parts do.
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