These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Transepithelial transport and metabolism of glycine in S1, S2, and S3 cell types of the rabbit proximal tubule.
    Author: Parks LD, Barfuss DW.
    Journal: Am J Physiol Renal Physiol; 2002 Dec; 283(6):F1208-15. PubMed ID: 12388405.
    Abstract:
    In the first of two sets of experiments, the lumen-to-cell and cell-to-bath transport rates for glycine were measured in the isolated-perfused medullary pars recta (S3 cells) of the rabbit proximal tubule at multiple luminal glycine concentrations (0-2.0 mM). The lumen-to-cell transport of glycine was saturated, which permitted the calculation of the transport maximum of disappearance rate of glycine from the lumen (pmol.min(-1).mm tubular length(-1)), K(m) (mM), and paracellular leak (pmol.min(-1).mm tubular length(-1).mM(-1)) values for this transport mechanism; these values were 4.3, 0.3, and 0.03, respectively. The cell-to-bath transport did not saturate but showed a linear relationship to cellular glycine concentration, 0.58 pmol.min(-1).mm tubular length(-1).mM(-1). The second set of experiments characterized the transport rate, cellular accumulation, and metabolic rate of lumen-to-cell transported [(3)H]glycine in all segments (cell types) of the proximal tubule, pars convoluta (S1 cells), cortical pars recta (S2 cells), and medullary pars recta (S3 cells). These proximal tubular segments were isolated and perfused at a single glycine concentration of 11.2 microM. From the results of this study and previous work (Barfuss DW and Schafer JA. Am J Physiol 236: F149-F162, 1979), we conclude that the axial heterogeneity for glycine lumen-to-cell and cell-to-bath transport capacity extends to the medullary pars recta (S3 cells; S1 > S2 < S3 for lumen-to-cell transport and S1 > S2 > S3 for cell-to-bath transport). Also, we conclude that lumen-to-cell transported glycine can be metabolized and its metabolic rate displays axial heterogeneity (S1 > S2 > S3). The physiological significances of these transport and metabolic characteristics of the S3 cell type permits the medullary pars recta to effectively recover glycine from very low luminal glycine concentrations and makes glycine available for protective and maintenance metabolism of the medullary pars recta.
    [Abstract] [Full Text] [Related] [New Search]