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  • Title: [Development of quantitative immunoenzyme and radioimmunologic analysis of lambda-free immunoglobulin light chains].
    Author: Piven' NV, Goncharik AV, Reznikov IuP, Shkumatova GA, Kul'bakova AI.
    Journal: Prikl Biokhim Mikrobiol; 2002; 38(5):578-84. PubMed ID: 12391762.
    Abstract:
    New quantitative techniques of radioimmunoassay (RIA) and enzyme immunoassay (EIA) were developed for determination of free light lambda-chains of immunoglobulins (immunometric sandwich-like variants) in biological fluids using two types of monoclonal specific antibodies (MAB-1 and MAB-2) to different epitopes of the antigen molecule: MAB-1 were immobilized on a solid phase (polystyrene beads), whereas MAB-2 were labeled with iodine or with the enzyme. The test systems prepared can be used for determination of concentrations from 25 to 1000 ng/ml, are very sensitive (25 ng/ml), and the analysis time is 5 h. The two methods were compared, and their clinical and diagnostic validity was evaluated in patients with various diseases associated with disorders in the antibody synthesis by the immune system.
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