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  • Title: Production of alpha-amylase with Aspergillus oryzae on spent brewing grain by solid substrate fermentation.
    Author: Bogar B, Szakacs G, Tengerdy RP, Linden JC, Pandey A.
    Journal: Appl Biochem Biotechnol; 2002; 102-103(1-6):453-61. PubMed ID: 12396145.
    Abstract:
    Ten Aspergillus oryzae strains were screened in solid substrate fermentation for alpha-amylase production on spent brewing grain (SBG) and on corn fiber. SBG proved to be a better substrate for enzyme production than corn fiber. A Plackett-Burman experimental design was used to optimize the medium composition for the best strain. Solid substrate fermentation on optimized medium with A. oryzae NRRL 1808 (=ATCC 12892) strain in stationary 500-mL Erlenmeyer flask culture yielded 4519 U of alpha-amylase/g of dry matter substrate in 3 d. The whole solid substrate fermentation material (crude enzyme, in situ enzyme) may be considered a cheap biocatalytic material for animal feed rations and for bioalcohol production from starchy materials.
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