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  • Title: [Biochemical changes in the myometrium of pregnant cats].
    Author: Zherev St.
    Journal: Eksp Med Morfol; 1975; 14(1):12-6. PubMed ID: 1240802.
    Abstract:
    The author carried out on 35 cats a study on the protein content, potasium and sodium. aldolase, GOT, GPT and LDH of the uterine muscle. The animals were divided into three groups: first-15 nonpregnant cats, second-10 pregnant cats at the first half of pregnancy and third-10 pregnant cats at the second half of pregnancy. He used a piece of uterine muscle from which proteins were extracted by solutions of potasium iodide with various strength. The total protein was determined by the method of Loury, but the sarcoplasmic proteins were examined electrophoretically. Electrolytes were estimated by flame photometer. The enzyme activity was examined by the reagents of "Boehringer". There was an increase of the amount of myosin and of the enzyme active sarcoplasmic protein fraction in the myometrium of pregnant animals. Potasium was increased in the uterine muscle during pregnancy, but sodium decreased. Enxyme activity of ALD, GOT and GPT was the highest in animals from the second group, but that of LDH-in the third group.
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