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  • Title: Determination of the antibacterial ofloxacin in human urine and serum samples by solid-phase spectrofluorimetry.
    Author: Ballesteros O, Vílchez JL, Navalón A.
    Journal: J Pharm Biomed Anal; 2002 Nov 07; 30(4):1103-10. PubMed ID: 12408901.
    Abstract:
    A method for the determination of trace amounts of ofloxacin has been developed, based on solid-phase spectrofluorimetry. The relative fluorescence intensity of ofloxacin fixed on Sephadex SP C-25 gel was measured directly after packing the gel beads in a 1-mm silica cell, using a solid-phase attachment. The wavelengths of excitation and emission were 294 and 494 nm, respectively. The linear concentration range of application was 0.5-16.0 ng ml(-1) of ofloxacin, with a relative standard deviation of 1.1% (for a level of 8.0 ng ml(-1)) and a detection limit of 0.14 ng ml(-1). The method was applied to the determination of ofloxacin in human urine and serum samples. It was validated applying the standard addition methodology and using HPLC as a reference method. Recovery levels of the method reached 100% in all cases.
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