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  • Title: Cyclic nucleotide content of tobacco BY-2 cells.
    Author: Richards H, Das S, Smith CJ, Pereira L, Geisbrecht A, Devitt NJ, Games DE, van Geyschem J, Gareth Brenton A, Newton RP.
    Journal: Phytochemistry; 2002 Nov; 61(5):531-7. PubMed ID: 12409019.
    Abstract:
    The cyclic nucleotide content of cultured tobacco bright yellow-2 (BY-2) cells was determined, after freeze-killing, perchlorate extraction and sequential chromatography, by radioimmunoassay. The identities of the putative cyclic nucleotides, adenosine 3',5'-cyclic monophosphate (cyclic AMP), guanosine 3',5'-cyclic monophosphate (cyclic GMP) and cytidine 3',5'-cyclic monophosphate (cyclic CMP) were unambiguously confirmed by tandem mass spectrometry. The potential of BY-2 cell cultures as a model system for future investigations of cyclic nucleotide function in higher plants is discussed.
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