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  • Title: Strand-specific loading of DnaB helicase by DnaA to a substrate mimicking unwound oriC.
    Author: Weigel C, Seitz H.
    Journal: Mol Microbiol; 2002 Nov; 46(4):1149-56. PubMed ID: 12421318.
    Abstract:
    We analysed the enzymatic activity (strand dis-placement) of the Escherichia coli DnaB helicase on a mirror-image pair of oligonucleotide-based substrates mimicking the unwound replication origin oriC. Loading of the helicase complex occurred exclusively to the single-stranded 'lower strand' part of the substrates. Full helicase activity required DnaA bound to the double-stranded part of the substrates (oriC DnaA box R1) and to their single-stranded 'upper strand' part. We assume that in vivo DnaA also loads the first of two helicase complexes - required for the assembly of two replication forks - to the lower strand of oriC during initiation of bidirectional chromosome replication in E. coli.
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