These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Preparative electrophoresis of peptidic glycosaminoglycans. Application to the fractionation of peptidic glycosaminoglycans of arterial wall (author's transl)].
    Author: Hermelin B, Deudon E, Bruel-Groleas A, Picard J.
    Journal: J Chromatogr; 1975 Jul 02; 110(1):43-52. PubMed ID: 124318.
    Abstract:
    A new procedure for the fractionation of glycosaminoglycans by electrophoresis on Pevikon has been described. Mixtures of glycosaminoglycans were fractionated by preparative electrophoresis on Pevikon in pyridine formate or glycine-HCl buffers. By this procedure, 200 mg of a mixture of hyaluronic acid, heparan sulphate, dermatan sulphate and chondroitin sulphate isolated from arterial wall could be successfully separated without loss of material. The purified fractions were analysed by enzymatic and chemical procedures. The molar ratios of uronic acid to hexosamine and of sulphate to hexosamine and the amino acid content of each glycosaminoglycan have been determined. The peptidic content (less than 1%) is represented by five amino acids, viz. serine, glycine, alanine, aspartic acid, and glutamic acid.
    [Abstract] [Full Text] [Related] [New Search]