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  • Title: Structure and function from the circadian clock protein KaiA of Synechococcus elongatus: a potential clock input mechanism.
    Author: Williams SB, Vakonakis I, Golden SS, LiWang AC.
    Journal: Proc Natl Acad Sci U S A; 2002 Nov 26; 99(24):15357-62. PubMed ID: 12438647.
    Abstract:
    In the cyanobacterium Synechococcus elongatus (PCC 7942) the proteins KaiA, KaiB, and KaiC are required for circadian clock function. We deduced a circadian clock function for KaiA from a combination of biochemical and structural data. Both KaiA and its isolated carboxyl-terminal domain (KaiA180C) stimulated KaiC autophosphorylation and facilitated attenuation of KaiC autophosphorylation by KaiB. An amino-terminal domain (KaiA135N) had no function in the autophosphorylation assay. NMR structure determination showed that KaiA135N is a pseudo-receiver domain. We propose that this pseudo-receiver is a timing input-device that regulates KaiA stimulation of KaiC autophosphorylation, which in turn is essential for circadian timekeeping.
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