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  • Title: Effect of time and temperature on blood P50 and 2,3-diphosphoglycerate measurements.
    Author: Hellerstein S, Bunthrarungroj T.
    Journal: Clin Chem; 1976 Jan; 22(1):39-41. PubMed ID: 1245059.
    Abstract:
    The oxygen affinity of hemoglobin is affected by various factors, including blood pH and erythrocyte 2,3-diphosphoglycerate. The latter and blood P50 values are unchanged when aliquots of blood from normal individuals are permitted to stand at room temperature for as long as 7 h. Our data also show that equilibration in a 37 degrees C tonometer for times ranging from 0.5 to 1.5 h causes no detectable alterations in P50 values.
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