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  • Title: Expression, crystallization and preliminary X-ray studies of the recombinant PTB domain of human dok-5 protein.
    Author: Shi N, Zhou W, Tang K, Gao Y, Jin J, Gao F, Peng X, Bartlam M, Qiang B, Yuan J, Rao Z.
    Journal: Acta Crystallogr D Biol Crystallogr; 2002 Dec; 58(Pt 12):2170-2. PubMed ID: 12454490.
    Abstract:
    The human dok-5 PTB domain fusion protein has been overexpressed in Escherichia coli and crystallized in a form suitable for X-ray crystallographic study. Crystals were obtained by the vapour-diffusion method. The crystal has unit-cell parameters a = b = 75.9, c = 108.0 A, alpha = beta = 90, gamma = 120 degrees and belongs to space group P3(2)21. Diffraction data were collected to 2.8 A resolution in-house. Furthermore, a selenomethionine (SeMet) derivative of dok-5 PTB domain fusion protein was overexpressed using the same expression system and was purified in a reductive environment. The derivative crystals were obtained under similar conditions. Subsequently, three different wavelength data sets were collected to 2.3 A resolution from the derivative crystal at the Advanced Photon Source, Argonne National Laboratory.
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