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  • Title: Preparation of prostatic plasma membranes Distribution of (Na-+, K-+)-ATPase and Mg-2+-ATPase in the rat ventral prostate.
    Author: Wilson MJ, Villee CA.
    Journal: Biochim Biophys Acta; 1975 Jun 11; 394(1):1-9. PubMed ID: 124595.
    Abstract:
    1. A method is described for the isolation of a plasma membrane fraction from rat ventral prostate. This fraction is greatly enriched in (Na-", K-+)-ATPase but also contains a small amount of Mg-2+-ATPase and 5'-nucleotidase activities. 2. The activity ratio (Mg-2+ plus Na-+ plus K-+/Mg-2+ or Mg-+ plus Na-+) of the plasma membrane (Na-+, K-+)-ATPase was 1.7. The enzyme system specifically requires ATP as substrate and is inhibited by Ca-2+ or ouabain.
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