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  • Title: Effect of application time of APF and NaF gels on microhardness and fluoride uptake of in vitro enamel caries.
    Author: Delbem AC, Cury JA.
    Journal: Am J Dent; 2002 Jun; 15(3):169-72. PubMed ID: 12469754.
    Abstract:
    PURPOSE: To evaluate the effect of time of fluoride application gel, acidulated or neutral, on in vitro enamel resistance to demineralization and fluoride uptake. MATERIALS AND METHODS: One hundred and ninety-two human enamel blocks were used in this study and 144 were treated with fluoride gel, acidulated or neutral, for 1 or 4 minutes. Ninety-six blocks treated with fluoride and 24 control blocks were submitted to a high cariogenic challenge. After the pH-cycling, enamel demineralization was assessed by surface and cross-sectional microhardness. Fluoride in the enamel blocks was also determined after removing an enamel layer by etching acid. RESULTS: Acidulated fluoride gel formed more fluoride in enamel than neutral gel (P<0.05), and it was also more efficient in reducing the demineralization of the enamel blocks submitted to a cariogenic challenge than the neutral one (P<0.05). It was found that the time of application was significant in terms of fluoride uptake, but it did not render the enamel more resistant to demineralization.
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