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  • Title: Rapid, preparative-scale purification of chromatin proteins.
    Author: Bhorjee JS, Pederson T.
    Journal: Biochim Biophys Acta; 1976 Jan 19; 418(2):154-9. PubMed ID: 1247540.
    Abstract:
    Methods are desceibed which permit rapid isolation of chromatographically purified histone and non-histone chromatin proteins under relatively mild chemical conditions. Chromatin is isolated from purified nuclei, dissociated in guanidine - HCl-urea and the nucleic acids removed by ultracentrigugation. This can be accomplished in 10 h by employing maximum-force rotors (500 000 x g). The proteins are then fractionated by a batch ion-exchange method, which leads to a rapid and complete separation of the histones and non-histone components, in apparently undegraded form. With these methods it is possible to obtain mg quantities of chromatographically pure histone and non-histone proteins in less than a single working day.
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