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  • Title: Interaction of distamycin A and netropsin with quadruplex and duplex structures: a comparative 1H-NMR study.
    Author: Randazzo A, Galeone A, Esposito V, Varra M, Mayol L.
    Journal: Nucleosides Nucleotides Nucleic Acids; 2002; 21(8-9):535-45. PubMed ID: 12484449.
    Abstract:
    Homonuclear NMR techniques have been used to investigate the interactions of the minor groove binding agents distamycin A (Dist-A) and the related drug netropsin (Net) with three quadruplexes characterized by different groove widths: [d(TGGGGT)]4 (Q1), [d(GGGGTTTTGGGG)]2 (Q2), and d(GGGGTTGGGGTGTGGGGTTGGGG) (Q3). Netropsin has been found to be in a fast chemical exchange with all three kinds of quadruplexes, whereas Dist-A interacts tightly with Q1 and, at a less extent, with Q2. In order to determine the degree of selectivity of Dist-A for two- rather than four-stranded DNA, we titrated with Dist-A an equimolar solution of Ql and the duplex d(CGCAAATTTGCG)2 (D). This comparative 1H-NMR study allowed us to conclude that Dist-A and, consequently, Net possess higher affinity for duplex DNA.
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