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  • Title: [The clinical significance of detection of p53 gene mutation in patients with lung cancer].
    Author: Chen Y, Cai Y, Liu L, Wang A, Wang J.
    Journal: Zhonghua Jie He He Hu Xi Za Zhi; 2002 Nov; 25(11):667-9. PubMed ID: 12490121.
    Abstract:
    OBJECTIVE: To assess the value of detection of p53 gene mutation in bronchial biopsy and sputum samples for the diagnosis of lung cancer. METHODS: Bronchial biopsy and sputum samples were collected from 120 patients confirmed to have lung cancer and 40 patients with benign pulmonary diseases. Polymerase chain reaction-single strand conformation polymorphism-AgNOS(3) staining method (PCR-SSCP) was performed for the detection of point mutation at exon 5 - 8 of the p53 gene. RESULTS: The positive rates of p53 point mutation in the carcinomatous tissue, the para-carcinomatous tissue, the contralateral bronchial tissue and the sputum from patients with lung cancer were 60% (36/60), 17% (10/60), 5% (3/60) and 43% (26/60), respectively (P < 0.05). In patients with benign pulmonary diseases, p53 point mutation in bronchial biopsy sample was detected in only one case (5%). The positive rate for the diagnosis of lung cancer increased from 48% (cytology alone) to 68% when the detection of p53 point mutation and cytological examination for sputum samples were combined. CONCLUSIONS: Detection of p53 gene mutation with PCR-SSCP-silver stain combined with cytological examination of sputum samples can improve the diagnosis of lung cancer.
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