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  • Title: Screening method for the detection of aflatoxin, ochratoxin, zearalenone, penicillic acid, and citrinin.
    Author: Wilson DM, Tabor WH, Trucksess MW.
    Journal: J Assoc Off Anal Chem; 1976 Jan; 59(1):125-7. PubMed ID: 1249024.
    Abstract:
    A modification of the official method for ochratoxins and a screening method for zearalenone, aflatoxin, and ochratoxin is described and expanded to include citrinin and penicillic acid. The method uses 0.5N phosphoric acidchloroform (1+10) in the initial extraction; the extract is divided and eluted from 2 columns to provide a quantitative thin layer chromatographic (TLC) method for aflatoxin and ochratoxin in corn and dried beans. Aflatoxin and zearalenone are eluted from one column and ochratoxin, penicillic acid, and citrinin from the other. Ochratoxin A recoveries are low (50%) in peanuts. Zearalenone, penicillic acid, and citrinin were qualitatively recovered from corn and beans; zearalenone and penicillic acid were recovered from peanuts but citrinin was not. Several TLC solvents were used to separate interferences.
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