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  • Title: Immunological reactions involving leukocytes: I. Detection of antibodies to human granulocytes by measurement of the metabolic events associated with phagocytosis.
    Author: Laleli YR, Bilezikian SB, Tsan MF, Hodkinson BA, McIntyre PA.
    Journal: Johns Hopkins Med J; 1976 Feb; 138(2):43-7. PubMed ID: 1249880.
    Abstract:
    During phagocytosis, glucose oxidation via the hexose monophosphate shunt (HMS) is markedly stimulated in human polymorphonuclear leukocytes. A method is described for the detection of antibodies to human granulocytes which exploits this fact. The source of antibodies was rabbit antihuman leukocyte serum (RAHLS) obtained by repeated immunization of normal rabbits with human granulocyte homogenates. HMS activity was quantitated by measuring the release of 14CO2 from glucose-1-14C. Under precisely defined experimental conditions, RAHLS and its IgG fraction inhibited 14CO2 release. The inhibitory effect of RAHLS on human granulocyte phagocytosis-associated HMS activity could be reduced by prior absorption of RAHLS with human granulocytes. Compared to the cytotoxic and leukoagglutination tests, this method appears more sensitive with respect to detection of xenogeneic antibodies to human granulocytes.
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