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  • Title: [Telomerase gene antisense oligodeoxynucleotide down-regulates telomerase activity in HL-60 cells].
    Author: He DM, Zhang H.
    Journal: Ai Zheng; 2002 Oct; 21(10):1070-4. PubMed ID: 12508645.
    Abstract:
    BACKGROUND & OBJECTIVE: Human telomerase reverse transcriptase (hTERT) is a limiting component for telomerase activity. Telomerase inhibitor targeting telomerase RNA could arrest growth of tumor cells. This study was designed to explore the effect of hTERT gene antisense phosphorothioate oligodeoxynucleotide (ASODN) on telomerase activity in HL-60 cells. METHODS: Telomeric repeat amplification protocol was used to determine telomerase activity. The expression levels of hTERT mRNA and protein were assayed by reverse transcriptase polymerase chain reaction (RT-PCR) and immunofluorescence using fluorescence isothiocyanate label respectively. RESULTS: RT-PCR and immunofluorescence assay showed that the expression levels of hTERT mRNA and protein from HL-60 cells decreased with time after hTERT ASODN treatment. There was no difference in hTERT mRNA and protein levels between control and SODN-treated cells. Telomerase activity decreased when HL-60 cells were treated with ASODN for 48 h. Telomerase activity of HL-60 cells was significantly inhibited when treated with ASODN for 72h. There was no difference in telomerase activity levels between control and hTERT SODN-treated cells. CONCLUSION: Telomerase activity could be down-regulated by hTERT ASODN in HL-60 cells.
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