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  • Title: [Use of diagnostic DNA probes for detection of the provirus of bovine leukemia virus in infected cows].
    Author: Mal'tseva NA, Kaledin AS, Oliĭnik EI, Zhivotov AA, Baranov VI, Karelin VP.
    Journal: Vopr Virusol; 2002; 47(6):38-41. PubMed ID: 12508683.
    Abstract:
    A method of detection of BLV proviral DNA in the peripheral blood leukocytes of cattle is reported. Leukocytes were used without preliminary cultivation. Cell DNA was dot-hybridized with P-labeled plasmid containing a fragment of BLV proviral DNA. The sera samples taken from the cattle under study were also tested using immunodiffusion assay (ID). The results of comparison showed that dot-hybridization assay is a more sensitive diagnostic test than ID, because it detects BLV infection in apparently normal cattle, which was seronegative in ID.
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