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  • Title: [Studies on separation and identification of Rhizoma coptidis brugs based on microemulsion thin-layer chromatography].
    Author: Kang C, Wen LY, Ding ZB, Guo R, Zhu XS.
    Journal: Zhongguo Zhong Yao Za Zhi; 2000 May; 25(5):262-5. PubMed ID: 12512445.
    Abstract:
    OBJECTIVE: To investigate the use of microemulsion TLC for the separation and identification of Rhizoma Coptidis drugs. METHOD: Thirteen Rhizoma Coptidis drugs were separated and identified with six kinds of SDS/n-C4H9OH/n-C7H16/H2O microemulsions as mobile phase on polyamide film. Effects of microemulsions on separation were investigated. RESULTS: The best mobile phase was the O/W microemulsion(containing 75% water). Compared with general mobile phases, the separation and detection could be improved and the test conditions were more tolerant. CONCLUSION: This new method is simple, accurate and efficient for the separation and identification of Rhizoma Coptidis drugs.
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