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  • Title: [Development of MTS/pms colorimetric assay in the proliferation of leukemic cells].
    Author: Chen XS, Fang TL, Cai RB, Guo GL.
    Journal: Zhongguo Shi Yan Xue Ye Xue Za Zhi; 2002 Oct; 10(5):438-40. PubMed ID: 12513744.
    Abstract:
    In order to establish a new more rapid, safe and sensitive colorimetric assay for the proliferation of leukemic cells, MTS/pms has been developed. This automated colorimetric assay is based on the characteristic of viable and metabolically active leukemic cells to cleave MTS/pms into a water-soluble product whose optical density is determined at 492 nm by an automated microtiter-plate reader photometer. The results indicated that only active leukemic cells cleaved MTS/pms into product measured, and dead cells did not reduce MTS/pms. A linear relations hip were found between the viable cell number and optical density of MTS/pms cleaved by HL-60 and K562 cell (r = 0.963). Compared with MTT and INT assays, the reduced product of MTS/pms is water-soluble. It is concluded that MTS/pms colorimetric assay is more rapid, accurate and sensitive for the bioassay of proliferation of leukemic cells.
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