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  • Title: [Studies on method for the determination of kaerophyllin in Bupleurum smithii wolff Wolff var. parvifolium].
    Author: Dou HS, Zeng SX, Wang YH, Shang MY.
    Journal: Zhongguo Zhong Yao Za Zhi; 2000 Aug; 25(8):488-9. PubMed ID: 12515213.
    Abstract:
    OBJECTIVE: To establish a quantitative method for the determination of kaerophyllin in Chinese medicine Bupleurum smithii ver. parvifolium. METHOD: Kaerophyllin was determined by HPLC, the mobile phase consisted of methanol-water(70:30), the flow rate was 1 ml.min-1, the eluate was monitored with UV detector at 328 nm. RESULT: The average recovery was 97.1% with RSD = 2.15 (n = 5). CONCLUSION: The result was precise and stable. This method was sensitive and its repeatability was excellent. This method may serve determination of kaerophyllin in B. smithii var. parvifolium.
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