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  • Title: The cryopreservation of Chlorella. 1. Interactions of rate of cooling, protective additive and warming rate.
    Author: Morris GJ.
    Journal: Arch Microbiol; 1976 Feb; 107(1):57-62. PubMed ID: 1252089.
    Abstract:
    The cryoprotective additives glycerol and dimethylsulphoxide were found to be toxic to Chlorella cells at concentrations greater then 2.5% w/v. Polyvinylpyrrolidone, was not damaging up to a concentration of 15%w/v. Chlorella 211/7a had a recovery rate greater than 95% at all rates of cooling studied. With Chlorella 211/8h the survival was lower than 0.1% at all rates examined. The addition of dimethylsulphoxide (5% w/v) to Chlorella 211/8h increased the recovery, particularly at the faster rates of cooling; with polyvinylpyrrolidone (10% w/v) there was an optimum range of cooling rate. Cells of Chlorella 211/7a from the exponential phase of growth were found to be damaged both by a temperature reduction from 25 degrees C to 0 degrees C (thermal shock) and by freezing and thawing. In contrast cells from the stationary phase of growth were resistant to these stresses.
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