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  • Title: [Preliminary study on apoptosis of human leukemic cells induced by bufalin].
    Author: Xu RC, Chen XY, Chen L, Qian J.
    Journal: Zhongguo Zhong Yao Za Zhi; 2001 Jan; 26(1):59-61. PubMed ID: 12525125.
    Abstract:
    OBJECTIVE: To study the mechanism of inhibiting actions of bufalin on human leukemic cells (HL60). METHODS: HL60 cells were incubated with bufalin at different concentrations and growth inhibition was analyzed by trypan-blue staining. Cell apoptosis was evaluated by phase contrast microscopy, transmission eletron microscopy and agarose gel electrophoresis. RESULTS: Bufalin remarkably inhibited the growth of HL60 cells. Apoptosis of HL60 cells could be effectively induced by bufalin at concentration of 0.01 mumol.L-1 or higher, showing the apoptotic changes in morphology, including cell shrinkage, chromatin agglutination and formation of apoptotic corpuscula; DNA cleavage was observed with agarose gel electrophoresis. CONCLUSION: Bufalin can effectively induce apoptosis of HL60 cells, which is possibly one of the mechanisms for anti-cancer of bufalin.
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