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  • Title: [Investigation of group A rotavirus infection in several cities in China and prevalence of VP7 serotype].
    Author: Xu S, Wen L, Wang J.
    Journal: Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi; 2001 Mar; 15(1):77-80. PubMed ID: 12526310.
    Abstract:
    OBJECTIVE: To study the prevalence of Group A rotavirus infection in China. METHODS: 374 samples from infants with diarrhea were collected from six cities (Beijing, Shenyang, Xinxiang, Shanghai, Shenzhen and Guangzhou) in autumn and winters of 1997 and/or 1998. The incidence of rotavirus infection and the prevalence of the four dominant serotypes G1, G2, G3, G4(VP7 serotype) were detected by PAGE and RT-PCR. RESULTS: Of the 374 samples detected, 181 were rotavirus positive. The positive rate was 48.4%, ranging from 32%-63% in different cities. The positive rates between 1997 and 1998 in Beijing and Shanghai were similar. Serotyped by RT-PCR method based on VP7 sequence, the positive rates of type G1, G2, G3 and G4 was 89.5%, 6.1%, 2.8% and 0, respectively. Two cases were found being coinfected with G1 and G2, and one case was confirmed to be infected with G9 by cDNA sequence analysis. There was a little difference in prevalence of G type rotavirus infection among samples from different cities and different years. CONCLUSION: The results indicated that rotavirus was the most important etiologic agent in this country, and the predominant serotypes were G1, G2 and G3.
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