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  • Title: Click chemistry to construct fluorescent oligonucleotides for DNA sequencing.
    Author: Seo TS, Li Z, Ruparel H, Ju J.
    Journal: J Org Chem; 2003 Jan 24; 68(2):609-12. PubMed ID: 12530893.
    Abstract:
    "Click chemistry" 1,3-dipolar cycloaddition between alkynyl 6-carboxyfluorescein (FAM) and azido-labeled single-stranded (ss) DNA was carried out under aqueous conditions to produce FAM-labeled ssDNA in quantitative yield. The FAM-labeled ssDNA was successfully used as a primer to produce DNA sequencing products with single-base resolution in a capillary electrophoresis DNA sequencer with laser-induced fluorescence detection.
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