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  • Title: [Forensic application of reverse dot blot with biotin incorporation].
    Author: Jiang WB, Jiang XH.
    Journal: Fa Yi Xue Za Zhi; 2001 Feb; 17(1):12-4, 61. PubMed ID: 12533884.
    Abstract:
    A rapid and accurate reverse dot blot technique was successfully established. It could distinguish the 6 alleles of 0101, 0102, 0103, 0201, 0301 and 0401 of HLA-DQA locus. Alleles and genotype frequencies for HLA-DQA locus were determined in 200 unrelated individuals of northern Han population. The discrimination power (DP) was 0.928, heterozygocity (H) 0.75. By direct biotin incorperation, 1 ng genome DNA could be detected successfully. This method can be used for paternity test and individual identification in forensic science practice.
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