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  • Title: [Determination of aflatoxins in peanut by high performance liquid chromatography using immunoaffinity column clean-up and on-line electrochemical derivatization].
    Author: Zhang P, Zhang YB, Zhao WD, Li YB.
    Journal: Se Pu; 2000 Jan; 18(1):82-4. PubMed ID: 12541466.
    Abstract:
    A method for the determination of aflatoxin B1, B2, G1 and G2 in peanut by high performance liquid chromatography using immunoaffinity column clean-up and on-line electrochemical derivatization was developed. Sample was extracted with 80% methanol and the extract was passed through the IAC. Aflatoxins were retained by the monoclonal in the IAC and eluted with actonitrile. AFT B1 and G1 were derivatized with on-line electrochemical equipment Kobra Cell. The four toxins can be separated in 13 minutes and the detection limit was 0.1 microgram/kg for each toxin. The method has been applied to the determination of peanut sample. The relative standard deviation was 9.2%-15% and the average recovery of AFT spiked at 0.5-9.0 micrograms/kg levels was in the range of 74.8%-97.3%.
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