These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Determination of berberine in decocted liquid from shenshu granules with water by reversed-phase liquid chromatography].
    Author: Wang SD, Song BS, Li K.
    Journal: Se Pu; 2000 May; 18(3):261-2. PubMed ID: 12541570.
    Abstract:
    A reversed-phase liquid chromatographic method has been developed and validated for the analysis of berberine in decocted liquid from Shenshu granules with water. The drug was extracted with supersonic wave and then measured by HPLC using a Hypersil C18 column and an acetonitrile -0.04 mol/L H3PO4(42:58, V/V) as mobile phase. Berberin was quantified by ultraviolet absorbance at 349 nm. The method proved to be linear in the range of 1.2-19.2 mg/L. The lower limit of detection of berberin was 0.6 mg/L. Intra-day and inter-day coefficients of variation of assay for berberin were 0.6%-3.5%(n = 5) and 5.3%-6.5% (n = 5), respectively. The recoveries of the method were 89.1%-91.4%.
    [Abstract] [Full Text] [Related] [New Search]