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  • Title: [Separation of different phosphatidyl cholines by reversed-phase ion-pair high performance liquid chromatography].
    Author: Wang ZH, Lu XQ, Hong XK.
    Journal: Se Pu; 2002 May; 20(3):249-52. PubMed ID: 12541948.
    Abstract:
    A reversed-phase ion-pair high performance liquid chromatographic (RP-IP-HPLC) method for the separation of different phosphatidyl cholines (PC) was established. The optimized conditions were a PERKIN-ELMER/HS-5 C18 column (150 mm x 4.6 mm i.d., 5 microns), isocratic elution with mobile phase of methanol-acetonitrile-water (70:22:8, volume ratio) containing 15 mmol/L tetramethyl ammonium phosphate (TMAP) at pH 7.0, flow rate of 2.0 mL/min and UV detection at 208 nm. Seven kinds of PC could be separated with this method.
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