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  • Title: Molecular identification of Paracoccidioides brasiliensis by 5' nuclease assay.
    Author: Semighini CP, de Camargo ZP, Puccia R, Goldman MH, Goldman GH.
    Journal: Diagn Microbiol Infect Dis; 2002 Dec; 44(4):383-6. PubMed ID: 12543545.
    Abstract:
    A PCR assay based on the 5' nuclease assay using a fluorescent probe derived from the sequence of the gene coding for the 43,000-Da (gp43) antigen was developed to detect Paracoccidioides brasiliensis. The assay could detect at least 10 copies of this DNA sequence, providing efficient accuracy to be useful for diagnosis of paracoccidioidomycosis.
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