These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Quantitative analysis of sparfloxacin injection by high performance liquid chromatography].
    Author: Cao SX, Zhang JY, Ji XM, Liu HM.
    Journal: Se Pu; 2001 Sep; 19(5):454-6. PubMed ID: 12545445.
    Abstract:
    Analytical method of the quantitative determination of sparfloxacin injection by HPLC is described. The analytical conditions were as follows. A Waters Symmetry C18(5 microns, 150 mm x 3.9 mm i.d.) column was used as the analytical column. The detection wavelength was UV-298.8 nm. The column temperature was 30 degrees C. The mobile phase was 0.2% KH2PO4 buffer (pH 3.2)-CH3CN-CH3OH (80:15:5, volume ratio) and the flow-rate was 1.0 mL/min. The injection volume was 10 microL. The linear range (the peak area vs. the mass concentration of sparfloxacin) was from 39.94 mg/L to 199.68 mg/L, and the correlation coefficient was 0.9999. The average recovery of sparfloxacin was 100.1% (n = 5), and its RSD was 0.72%. The RSDs of continuous injections, within day injections per 2 hours and between day injections in three days were 0.19%, 0.14% and 0.13% respectively. The above analytical results show that this method has good precision and stability. It is a rapid, sensitive and accurate method for the analysis of sparfloxacin.
    [Abstract] [Full Text] [Related] [New Search]