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  • Title: [Study on solid phase extraction spectrophotometric determination of copper(II) with 2-(4-antipyrinylazo)-5-dimethylaminoaniline].
    Author: Li Y, Liu S, Yin J.
    Journal: Wei Sheng Yan Jiu; 2002 Jun; 31(3):203-4. PubMed ID: 12545763.
    Abstract:
    Based on the color reaction of 2-(4-Antipyrinylazo)-5-Dimethylaminoaniline (ADA) with copper (II) and the solid phase extraction of its colored complex with Waters Porapak(r) Sep-Park-C18 cartridge, a new method for the determination trace amount of copper(II) was studied. In the medium of Tween-80 and presence of pH = 4.5 buffer solution, ADA can react with copper (II) to form a stable 2:1 complex. The colored complex can extracted by Porapak(r) Sep-Park-C18 cartridge and eluted by ethanol contain 1% of acetic acid, then can be determined by spectrophotometer at 530 nm. Beer's law is obeyed in range of 0.05(1.0(g/ml. This method can be applied to the determination trace amount of copper in water with satisfactory results.
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