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  • Title: Carbohydrate composition of bovine rhodopsin.
    Author: Plantner JJ, Kean EL.
    Journal: J Biol Chem; 1976 Mar 25; 251(6):1548-52. PubMed ID: 1254583.
    Abstract:
    The carbohydrate content of bovine rhodopsin was investigated and found to be different from previously reported values. Rod outer segments were isolated from dark-adapted bovine retinas by sucrose flotation and purified by sucrose density contrifugation. Rhodopsin was extracted with detergents and purified by chromatographic procedures involving calcium phosphate/celite chromatography followed by affinity chromatograpy on concanavalin A-Sepharose (or in some cases, gel filtration on agarose). Purified preparations of rhodopsin had A278/A498 ratios of 1.6 to 2.0. After treatment of the rhodopsin with chloroform/methanol (2/1) to remove lipids and detergents, the carbohydrate content was measured by gas-liquid chromatography, colorimetric and enzymatic analyses, paper chromatography, and electrophoresis. Rhodopsin was found to have about 9 mol of mannose and 5 mol of glucosamine per mol of visual pigment. A molar ratio of mannose/glucosamine of about 2 was also found in samples of rhodopsin obtained from two other laboratories. The amino acid analysis was similar to previously published values.
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