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  • Title: [Expression and insecticidal characteristics of cry3A in Lepidoptera-specific Bacillus thuringiensis].
    Author: Yue C, Liu Z, Zeng X, Shao Z, Yu Z.
    Journal: Wei Sheng Wu Xue Bao; 2000 Apr; 40(2):139-42. PubMed ID: 12548935.
    Abstract:
    The cry3A gene coding Coleoptera-specific protein was transformed into a wild strain YBT803-1 by electroporation, A transformant BMBY-001 was obtained. SDS-PAGE analysis demonstrated that cry3A could express 64 kD protein in BMBY-001. However, the expression of cry1Ab and cry1Ac were effected. Bioassay showed that BMBY-001 was not only highly toxic to Phyllodecta vulgatissima larva(LC50 0.413 microL/mL) but also toxic to Plutella xylostella(LC50 3.319 microL/mL).
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