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  • Title: [Purification and properties of degrading enzyme of organophosphorus pesticides from Aspergillus ornatus Z58].
    Author: Liu Y, Zhong Y.
    Journal: Wei Sheng Wu Xue Bao; 2000 Aug; 40(4):430-4. PubMed ID: 12548967.
    Abstract:
    Degrading enzyme of organophosophorus pesticides was purified from Aspergillus oryzeZ58 by (NH4)2SO4 precipitation, Sephadex G-100 gel filtration, DEAE-cellulose chromatography with 34.2-fold purification, 17.8% recovery and 76.2 U/mg specific activity. The purified enzyme was homogeneous on polyacrylamide gel electrophoresis. The molecular weight of the enzyme estimated with Sephadex G-100 was 67000. The optimal conditions for activity were pH 7.2, temperature 45 degrees C. The enzyme was stable over the range of pH 6-10 and below 40 degrees C, the activity was inhibited by SDS and stimulated by Cu2+, the substrate ranges were nonspecific to organophosophorus pesticides.
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