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Title: [Determination of alpha-acid in hop by high performance liquid chromatography]. Author: Chen J, Shen L. Journal: Se Pu; 1999 Mar; 17(2):184-6. PubMed ID: 12549164. Abstract: A method for the determination of alpha-acid in hop by reversed-phase high performance liquid chromatgraphy is presented. The alpha-acid in hop and hop exteact was separated in a mu-Bondapak C18 cartridge by using V(CH3OH):V(H2O):V(H3PO4) = 80:20:0.25 as mobile phase. The flow rate was 1.0 mL/min and detection of alpha-acid was performed with UV detector at 314 nm. Quantification of alpha-acid was carried out by measuring peak height/peak area and comparing with external standard. The relative standard deviation of alpha-acid in hop was 2.02% and in hop extract was 1.78%. The method is simple, rapid and satisfactory.[Abstract] [Full Text] [Related] [New Search]