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  • Title: [Construction of plasmid gene bank of V. cholerae O139 and detection of O-antigen genes].
    Author: Qu D, Liu C, Zhang Y, Ma Q.
    Journal: Wei Sheng Wu Xue Bao; 2001 Feb; 41(1):65-9. PubMed ID: 12549191.
    Abstract:
    Because O-antigen biosynthesis genes are a tandem gene cluster. Gnomic fragments of 4-20 kilobases (kb) were obtained by digesting genomic DNA of V. cholerae O139 with restriction endonuclease EcoRI, then plasmid gene bank was constructed. Recombinant colony, E. coli DH5 alpha (pMG320), expressing O-antigen of V. cholerae O139 was detected from the bank by immunological agglutinative reaction. The futher analysis showed O-antigen expressed by recombinant colony had both immunogenicity and reactogenicity, and the size of O-antigen biosynthesis genes was about 4.6 kb.
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